Table 1. Sequences of primers used for quantitative real-time PCR.
Gene | Oligonucleotide sequence | Product size (bp) | |
---|---|---|---|
Forward (5′–3′) | Reverse (5′–3′) | ||
SOX9 | ATGAAGATGACCGACGAGCA | CAGTCGTAGCCTTTGAGCAC | 236 |
Type II collagen | AGAACTGGTGGAGCAGCAAGA | AGCAGGCGTAGGAAGGTCAT | 142 |
Aggrecan | TGAGCGGCAGCACTTTGAC | TGAGTACAGGAGGCTTGAGG | 287 |
MMP3 | TTCCTTGGATTGGAGGTGAC | AGCCTGGAGAATGTGAGTGG | 248 |
MMP13 | CCCAACCCTAAACATCCAA | AAACAGCTCCGCATCAACC | 147 |
ADAMTS4 | ACCCAAGCATCCGCAATC | TGCCCACATCAGCCATAC | 246 |
ADAMTS5 | GACAGTTCAAAGCCAAAGACC | TTTCCTTCGTGGCAGAGT | 204 |
Bcl-2 | GCCTTCTTTGAGTTCGGTGG | GAAATCAAACAGAGGCCGCA | 192 |
U6 | CGCTTCGGCAGCACATATAC | AAATATGGAACGCTTCACGA | 100 |
β-actin | AGCGAGCATCCCCCAAAGTT | GGGCACGAAGGCTCATCATT | 285 |