Figure 1. Pygo2 promotes the proliferation of glioma cells treated with paclitaxel.
Six groups of cells (PTX, PTX+ pHM6-Pygo2, PTX+Pygo2 shRNA, PTX+pHM6, PTX+scr shRNA) were seeded at a density of 1×105 cells/well in 6-well plates and treated with 30 nM PTX for 48 h; normal cells were used as a control. (A–B) An MTT assay revealed that Pygo2 counteracted the effect of PTX-induced cell growth arrest in human glioma U-87MG or U251 cell lines. (C, F) qRT-PCR showed the relative Pygo2 mRNA expression level in the treated cell lines. Pygo2 expression was normalized to β-actin. (D–E) Pygo2 rescued the cell viability of U-87MG and U251 cells. Data are presented as the mean ± SD of three independent experiments. Two-tailed Student's t-test was used; *P < 0.05 and **P < 0.01.