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. 2017 Mar 2;8(17):27953–27965. doi: 10.18632/oncotarget.15853

Figure 3. Polyploidy and apoptosis of HCC cells were induced by the inhibitors of Aurora kinases.

Figure 3

(A–B) Polyploidy (DNA>4N) of HepG2(A) and SMMC-7721(B) cells was induced by SNS-314 in a dose-dependent manner, which was showed by PI staining to determine the cell cycle profile. (C–D) Apoptosis of HepG2(C) and SMMC-7721(D) cells was induced by SNS-314 in a dose-dependent manner, which was showed by PI and Annexin V staining. (E–F) Expression of apoptotic proteins following SNS-314 treatment in HepG2(E) and SMMC-7721(F) cells suggested by WB analysis and quantified by the Image J software. (G–H) The expression of apoptotic proteins induced by SNS-314 was much higher than MLN8237 and AZD1152-HQPA in HepG2 cells which was suggested by WB analysis and quantified by the Image J software. FL, Fluorescence; C-PARP, cleaved-PARP; C-caspase3, cleaved- caspase3; Mean ± SD; **p < 0.01, ***p < 0.001.