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. 2017 Mar 2;8(17):27953–27965. doi: 10.18632/oncotarget.15853

Figure 5. Knockdown of P21 impaired the apoptotic effect of the HCC cell lines treated with SNS-314.

Figure 5

(A–B) P21 knockdown impaired the apoptosis of HepG2(A) and SMMC-7721(B) cells induced by SNS-314 with indicated concentrations for 96h, suggested by PI and Annexin V staining. (C–F) According to WB analysis, knockdown of P21 reduced the activation of cleaved-PARP and cleaved-caspase3, in addition, induced the accumulation of YAP and impaired the reduction of YAP induced by SNS-314 for 96h in HepG2 (C) and SMMC-7721 (D) and the expression of the proteins were quantified by the Image J software in HepG2 (E) and SMMC-7721 (F). FL, Fluorescence; C-PARP, cleaved-PARP; C-caspase3, cleaved- caspase3; Mean ± SD; *p < 0.05, **p < 0.01, ***p< 0.001.