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. 2017 Mar 3;8(17):28074–28092. doi: 10.18632/oncotarget.15888

Figure 1. Hypoxia induced dedifferentiation of single CD133−CD15−NESTIN− cell into neurospheres.

Figure 1

(A) Magnetic cell sorting (MACS) and hypoxia treatment strategy. Single CD133−CD15−NESTIN− cell sorted through MACS was transferred to each well of 96-well plates and incubated under 1% hypoxia or 21% normoxia. (B) Following 21 d of incubation, the surviving single CD133−CD15−NESTIN− glioma cell dedifferentiated to neurospheres under hypoxia but not normoxia. Arrow indicated seeded cell and corresponding spheroid formation. (C) The formation of neurospheres significantly increased under hypoxia compared with normoxia (Paired-samples T Test). (D) Trypan blue assay showed almost all the cells in neurospheres kept survival. (E) When hypoxia-induced neurospheres were cultured with stem cell medium, they maintained an undifferentiated sphere-like status. When cultured with 10% FBS, adherent growth and morphology were identified.