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. 2017 Mar 6;8(17):28116–28124. doi: 10.18632/oncotarget.15922

Figure 1. The effects of PARP1-i, HT, cDDP and combined treatments on BRCA2, Rad51 and cell survival are shown.

Figure 1

(A) Western blots demonstrating R1, SiHa and HeLa cells are BRCA2 proficient. After HT, BRCA2 is downregulated. (B) γ-H2AX and Rad51 co-localization, to investigate activity of homologous recombination. (C) Clonogenic assays were performed to study the effect of the different treatment combinations, 10–12 days after treatments. The addition of PARP1-i to cDDP-based thermochemotherapy resulted in a significantly lower cell survival compared to cDDP-based thermochemotherapy alone. R1: p = 0.0008, SiHa: p = 0.034, HeLa: p = 0.021. The bar graph shows the mean of at least five independent experiments. From left to right: R1, SiHa, Hela cells. *p < 0.05, **p < 0.01, ***p < 0.001.