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. 2017 Mar 8;8(17):28226–28236. doi: 10.18632/oncotarget.15999

Figure 3. miR-let-7a upregulation inhibited SiHa and HeLa cell proliferation, migration and invasion.

Figure 3

(A) Validation of miR-let-7a expression levels after transfection with miR-let-7a mimics, miR-let-7a inhibitor, or the relative controls by RT-PCR analysis. CCK-8 assays revealed that up-regulation of miR-let-7a inhibited cell proliferation of SiHa and HeLa cells. *denotes significance at P < 0.01 relative to NC miRNAs by ANOVA. (B–C) Transwell assay was conducted to identify the role of miR-let-7a in migration and invasion, and up-regulation of miR-let-7a inhibited SiHa and HeLa cell migration and invasion. Representative micrographs and quantification of crystal violet-stained cell colonies were > 0.1 mm. Cells that penetrated the membrane were photographed at 100× magnification. Each bar represents the mean of three independent experiments. *denotes significance at P < 0.01 relative to NC miRNAs by student t-test.