Table 2. Differentially expressed proteins of ‘TRG 1-2′ group related to rectal cancer (RC) in comparison with those of either ‘TRG 3′ or ‘TRG 4′ groups.
| Spot nr.a) | MW (Da)/pI | Accession | Gene | Protein annotation | Cellular locationb) | Fold Δ | p-value |
|---|---|---|---|---|---|---|---|
| Class a) Up-regulated spots in ‘TRG 1-2′ versus ‘TRG 3′ (nr = 5) | |||||||
| 114 | 99551/5.34 | MVP_HUMAN | MVP | Major vault protein | nucleus, nuclear pore complex | 2.2 | 0.023 |
| 150 | 84026/6.08 | IMMT_HUMAN | IMMT | Mitochondrial inner membrane protein or mitofilin | mitochondrion inner membrane | 1.7 | 0.048 |
| 152 | 82973/6.15 | gi|154354966 | IMMT | Mitochondrial inner membrane protein isoform 3 or mitofilin | mitochondrion inner membrane | 1.6 | 0.012 |
| 333 | 54541/5.61 | gi|220702506 | TAPBP, PDIA3 | Chain A, TapasinERP57 HETERODIMER | endoplasmic reticulum | 1.5 | 0.015 |
| 358 | 57794/6.01 | TCPB_HUMAN | CCT2 | T-complex protein 1 subunit ß | cytoplasm | 1.5 | 0.011 |
| Class b) Up-regulated spots in ‘TRG 1-2′ versus ‘TRG 4′ (nr = 3) | |||||||
| 908 | 18229/7.68 | PPIA_HUMAN | PPIA | Peptidyl-prolyl cis-trans isomerase or Cyclophilin A | cytoplasm, secreted | 2.8 | 0.046 |
| 289 | 79805/7.7 | gi|34228 | LMNA | Unnamed protein product, putative lamin A precursor | 1.8 | 0.010 | |
| 571 | 36088/5.84 | gi|1703319 | ANXA4 | Annexin A4 | cytoplasm, cell surface, membranes, nucleus, secreted | 1.7 | 0.010 |
| Class c) Up-regulated spots in ‘TRG 3′ versus ‘TRG 1-2′ (nr = 11) | |||||||
| 373 | 53548/6 | gi|148491091 | SLC25A24 | Calcium-binding mitochondrial carrier protein SCaMC-1 isoform 1 | mitochondrion inner membrane | −1.5 | 0.028 |
| 535 | 36892/6.32 | AK1A1_HUMAN | ADH1A | Alcohol dehydrogenase [NADP(+)] | cytoplasm, apical plasma membrane, extracellular exosome, extracellular space | −1.5 | 0.035 |
| 811 | 19871/4.80 | MYL9_HUMAN | MYL9 | Myosin regulatory light polypeptide 9 | cytoplasm, muscle myosin, stress fiber, Z disc | −1.5 | 0.019 |
| 377 | 59828/9.16 | ATPA_HUMAN | ATP5A1 | ATP synthase subunit α, mitochondrial | mitochondrion inner membrane, cell membrane, peripheral membrane protein, extracellular side | −1.6 | 0.044 |
| 471 | 56577/8.54 | FIBB_HUMAN | FGB | Fibrinogen ß chain | secreted | −1.6 | 0.022 |
| 42052/5.29 | ACTB_HUMAN | ACTB | Actin, cytoplasmic 1 | cytoplasm, cytoskeleton | |||
| 43004/5.61 | SPB9_HUMAN | SERPINB9 | Serpin B9 | cytoplasm | |||
| 42530/5.72 | SPB5_HUMAN | SERPINB5 | Serpin B5 | cytoplasm | |||
| 580 | 32856/4.72 | gi|63252900 | TPM1 | Tropomyosin α-1 chain isoform 4 | cytoplasm, cytoskeleton | −1.9 | 0.013 |
| 284 | 59947/6.90 | CATA_HUMAN | CAT | Catalase | peroxisome | −2.1 | 0.016 |
| 573 | 37688 | TALDO_HUMAN | TALDO1 | Transaldolase | cytoplasm | −2.1 | 0.005 |
| 492 | 56577/8.54 | FIBB_HUMAN | FGB | Fibrinogen ß chain | secreted | −2.2 | 0.002 |
| 42052/5.29 | ACTB_HUMAN | ACTB | Actin, cytoplasmic 1 | cytoplasm, cytoskeleton | |||
| 42540/5.72 | gi|60817455 | SERPINB5 | Serpin B5 | cytoplasm | |||
| 683 | 42052/5.29 | ACTB_HUMAN | ACTB | Actin, cytoplasmic 1 | cytoplasm, cytoskeleton | −2.3 | 0.003 |
| 30749/5.86 | PRDX4_HUMAN | PRDX4 | Peroxiredoxin-4 | cytoplasm, secreted | |||
| 45037/6.10 | CATD_HUMAN | CTSD | Cathepsin D | secreted, extracellular space | |||
| 684 | 42052/5.29 | ACTB_HUMAN | ACTB | Actin, cytoplasmic 1 | cytoplasm, cytoskeleton | −2.8 | 0.003 |
| 45037/6.10 | CATD_HUMAN | CTSD | Cathepsin D | secreted, extracellular space | |||
| Class d) Up-regulated spots in ‘TRG 4′ versus ‘TRG 1-2′ (n = 11) | |||||||
| 193 | 74380/6.57 | LMNA_HUMAN | LMNA | Prelamin-A/C | nucleus (nucleoplasm, lamina, envelope) | −1.3 | 0.030 |
| 79294/6.81 | TRFE_HUMAN | TF | Serotransferrin | secreted | |||
| 342 | 56525/5.26 | ATPB_HUMAN | ATP5B | ATP synthase subunit ß, mitochondrial | mitochondrion inner membrane, cell membrane, peripheral membrane protein | −1.6 | 0.021 |
| 50095/4.78 | TBB5_HUMAN | TUBB | Tubulin ß chain | cytoplasm | |||
| 264 | 68988/5.69 | gi|157830361 | ALB | Chain A, Human Serum Albumin In A Complex With Myristic Acid And Tri- Iodobenzoic Acid | extracellular space | −1.7 | 0.025 |
| 471 | 56577/8.54 | FIBB_HUMAN | FGB | Fibrinogen ß chain | secreted | −1.9 | 0.009 |
| 42052/5.29 | ACTB_HUMAN | ACTB | Actin, cytoplasmic 1 | cytoplasm, cytoskeleton | |||
| 43004/5.61 | SPB9_HUMAN | SERPINB9 | Serpin B9 | cytoplasm | |||
| 683 | 42052/5.29 | ACTB_HUMAN | ACTB | Actin, cytoplasmic 1 | cytoplasm, cytoskeleton | −1.9 | 0.040 |
| 30749/5.86 | PRDX4_HUMAN | PRDX4 | Peroxiredoxin-4 | cytoplasm, secreted | |||
| 45037/6.10 | CATD_HUMAN | CTSD | Cathepsin D | secreted, extracellular space | |||
| 425 | 42215/5.91 | ACTBM_HUMAN | POTEI | Putative ß-actin-like protein 3 (POTE ankyrin domain family member K) | cytoplasm, cytoskeleton | −2.3 | 0.019 |
| 741 | 23883/5.35 | gi|194173391 | Immunoglobulin light chain | secreted | −2.4 | 0.043 | |
| 553 | 34726/6.06 | PSDE_HUMAN | PSMD14 | 26S proteasome non-ATPase regulatory subunit 14 | cytoplasm, proteasome | −2.5 | 0.035 |
| 684 | 42052/5.29 | ACTB_HUMAN | ACTB | Actin, cytoplasmic 1 | cytoplasm, cytoskeleton | −2.5 | 0.031 |
| 45037/6.10 | CATD_HUMAN | CTSD | Cathepsin D | secreted, extracellular space | |||
| 377 | 59828/9.16 | ATPA_HUMAN | ATP5A1 | ATP synthase subunit α, mitochondrial | mitochondrion inner membrane, cell membrane, peripheral membrane protein, extracellular side | −2.7 | 0.008 |
| 828 | 20876/8.69 | gi|62897565 | TAGLN | Transgelin variant | cytoplasm | −3.8 | 0.050 |
a)spot nr., spot numbers refer to Figure 2; b)keywords of cellular location from UniProtKB (http://www.uniprot.org/uniprot/) are reported.