Skip to main content
. 2017 Mar 10;8(17):28431–28441. doi: 10.18632/oncotarget.16085

Figure 5. Effects of wogonin and DAPT on neural differentiation of BMSCs.

Figure 5

BMSCs from the different experimental groups were treated with or without DAPT/wogonin combination and subjected to RT-PCR and western blotting analyses. (A) mRNA levels of Nestin, Pax6 and Oct4 examined by RT-PCR. Densitometric analysis of the effect of wogonin on Nestin, Pax6 and Oct4 protein expression (B). Protein levels of glial markers (O4, CD11b and GFAP) examined by western blot. (C) PKCα, rhodopsin, and RPE65 levels assessed by western blot. (D) NeuN, Brn3a, and Thy-1 protein expression assessed by western blot. β-actin was used to ensure equal loading in all groups. Data are shown as mean ± SEM (n = 6 per group, #p < 0.05, DAPT or wogonin versus neural induction group, *p < 0.01, wogonin-plus-DAPT versus DAPT group).