Table 1. Upregulation of HLA and costimulatory molecules by IFN-gamma pretreatment.

Flow Cytometric evaluation of markers upregulate by IFN-gamma treatment of EC
Placenta Number	Treatment	HLA 1	HLA 2	CD40	CD80	CD86
1	Control	ND	+	+	+	ND
1	50 U	++	++	+++	++	+++
1	100 U	+++	++	+++	++	+++
2	Control	ND	ND	ND	+	ND
2	50 U	++	++	+++	++	+
2	100 U	+++	+++	+++	++	++
3	Control	ND	ND	ND	+	ND
3	50 U	++	++	+++	++	+
3	100 U	+++	+++	+++	++	++
4	Control	ND	ND	ND	+	ND
4	50 U	+++	+++	+++	+++	+
4	100 U	+++	+++	+++	+++	++
5	Control	ND	ND	ND	+	ND
5	50 U	++	+++	+++	++	+
5	100 U	+++	+++	+++	++	++

Placental endothelial cells were isolated from 5 different donors and treated for 48 hours with dilution buffer (control) 50 or 100 IU interferon gamma. Flow cytometry was utilized to assess expression of HLA I, HLA II, as well as costimulatory molecules CD40, CD80, and CD86. Quantification was performed by mean fluorescent intensity. ND = Not Detectable compare to isotype control; + = >25% MFI compared to isotype control; ++ = > 50% MFI compared to isotype control; +++ = > 75% MFI compared to isotype control; Experiments were done in triplicate in 3 different flow cytometry runs.