Table 2. Sequence of oligonucleotide primers used for the analysis of TCF7L2 polymorphisms.
SNP-ID | 1st-PCRP | 2nd-PCRP | UEP |
---|---|---|---|
rs12573128 | ACGTTGGATGCAGGTAACT TGCTCAAGAGG | ACGTTGGATGCATTCTTCTG AGGTATGGAC | TGGACTTAAATTAGCT AATTAGG |
rs7081062 | ACGTTGGATGCCTTTAAGT TCTCTTTCAAG | ACGTTGGATGTGAAGAAGCC AAGAGTTTCC | AGAGTTTCCTGTTAATT AAAAAGA |
rs4918789 | ACGTTGGATGCAAAAGGCA AGGCGATTTTC | ACGTTGGATGCATGGTGTAC AACTCACACT | TTTGCTCTCTACA CCCTCA |
rs3750804 | ACGTTGGATGAGAAAGGTG CCAGCTTCAAC | ACGTTGGATGTTTCTGGGGC GGTCGCAGG | aCGCAGGCTGACTAACA |
rs6585205 | ACGTTGGATGTATTGACCC AACTTGGTCCC | ACGTTGGATGCTCTTGCCAT TCCTGGTTTC | actgGCCATTCCTGGTTTCA TCTAAGT |
rs290489 | ACGTTGGATGTGCTGTCCC CAGCTTCTTTC | ACGTTGGATGCTTGACCTGT CTTTCCAGGC | TTCCAGGCCCTTCTC |
Abbreviations: SNP: Single nucleotide polymorphism; PCRP: Polymerase chain reaction primer; UEP: Unique base extension primer.
Sequences are written in the 5′→3′ (left to right) orientation.