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. 2017 Feb 23;8(17):28683–28695. doi: 10.18632/oncotarget.15640

Figure 4. TRAF6 is a direct downstream target of miR-146b-5p in HCC.

Figure 4

A. The assumed miR-146b-5p binding sequences in the 3′-UTR of TRAF6. B. Wild-type miR-146b-5p crippled the luciferase activity that carried the 3′-UTR of TRAF6 mRNA. ***P<0.001. C-F. Alteration of miR-146b-5p levels regulated TRAF6 expression in vitro (C and D) and in vivo (E and F). The mRNA (C and E) and protein (D and F) expression levels of TRAF6 were down-regulated through miR-146b-5p over-expression and up-regulated by miR-146b-5p knockdown. The mRNA levels were determined by qRT-PCR. The protein expression was detected by immunoblotting in vitro and IHC in vivo. *P<0.01. G. TRAF6 expression was lower in high miR-146b-5p group than that in low miR-146b-5p group. *P<0.01. All experiments were performed at least in triplicate and the data in B-G are presented as the (mean ± SD).