Figure 4. After Nrf2 was knocked down by siRNA-Nrf2 (1), the apoptosis rates of NC and WT cells were tested by flow cytometry again.

After Nrf2 expression was reduced by siRNA-Nrf2, WT and NC, overexpressing U87 cells (A) and U251 cells (B) were treated with 400 μM TMZ for 72 h. The cells apoptosis was significantly increased. ***P < 0.001 compared with NC, WT.