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. 2017 Mar 7;8(17):29013–29027. doi: 10.18632/oncotarget.15994

Figure 3. α2,3-sialylation inhibitor SsaI suppresses ovarian cancer tumor migration and peritoneal dissemination.

Figure 3

(A) Quantification of ST3GalI in ovarian cancer cells (MOSEC, ES2, and OVCAR3) treated with 100μM SsaI or DMSO control for 72h by western blot; GAPDH was used as internal control. (B) The Transwell migration (upper panel) and invasion assay (low panel) on ovarian cancer cells that were stained with Toluidine blue and Crystal violet solution, respectively. Data shown are the mean ± SD of 3 separate experiments. (C) MOSEC cells (2×106) were injected into the peritoneal cavity of 8weekold female C57BL/6 mice (n=10). ALZET Micro-Osmotic Pumps were filled with either 100μM SsaI or DMSO as control was implanted subcutaneously. The mice were sacrificed after 4 weeks and the amount of ascites was measured. The body weights of mice were recorded after injecting MOSEC cells and implantation of pumps implantation. Data shown are the mean ± SD of 5 mice. Peritoneal seeding and carcinomatosis were analyzed in the SsaI and DMSO control groups. (*: p< 0.05, **: p<0.01, ***: p< 0.001.)