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. 2017 Mar 10;8(17):29056–29066. doi: 10.18632/oncotarget.16072

Figure 7. KIF3A acts as a downstream mediator of PLCD1.

Figure 7

BT-549 cells transfected with siN.C. or siPLCD1 were also transfected simultaneously with (or without) siKIF3A for 72 h. (A) Cell migration assays. Confluent cell monolayers were scratched and migration distance was measured over a 16 h period by phase-contrast microscopy. Representative wound-healing assays are shown on the left. Means ± SD, **p<0.01. (B) Cell invasion assays. BT-549 cells migrated to the lower side of the chamber or invaded through the Matrigel barrier. Cells were fixed, stained and counted using an inverted microscope. Five random fields were taken for each chamber. Representative cell migration and cell invasion assays are shown on the left. Means ±SD, **p<0.01. (C) Immunoblotting of KIF3A, pERK1/2, pGSK-3β, active-β-catenin, and MMP7 protein levels with β-actin as an internal control. Supernatant MMP7 levels were also measured by ELISA. Data were based on at least three independent assays, and representative images were shown. Means ± SD, **p<0.01.