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. 2017 Mar 22;8(17):29328–29345. doi: 10.18632/oncotarget.16440

Figure 5. ICI and si-ERα reversed the growth inhibition of Combinatorial Z-LIG and TAM in MDA-MB-231 cells.

Figure 5

(A) MDA-MB-231 cells were pre-treated with 100 nM ICI182780 or vehicle for 12 h, and then with or without Z-LIG (50 μM) for another 72 h. ERα expression was analyzed by Western blotting. (B) The cell viability was detected by SRB assay. Cells were pre-treated with vehicle or different concentrations of ICI (10,100,1000 nM) for 12h and then treated with or without Z-LIG (50 μM) combined with TAM (5 μM) for another 72h. (C) The expression of ERα was measured by Western blotting in si-NC or si-ERα-transfected MDA-MB-231 cells treated with or without Z-LIG (50 μM) for 72 h. (D) The cell viability was detected by SRB assay after MDA-MB-231 cells were transfected with si-NC or si-ERα, and then treated with or without Z-LIG (50 μM) combined with TAM (5 μM) for another 72h. (E) Colony formation assay was performed in MDA-MB-231 cells that first transfected with si-NC or si-ERα, and then treated with combinatorial Z-LIG (25 μM) and TAM (2.5 μM) and allowed to grow for two weeks before stained with 0.005% crystal violet. Values represent mean ± SD. The blots or images were a representative of three independent experiments. *p< 0.05, **p< 0.01 compared with control.