Skip to main content
. 2017 Mar 22;8(17):29328–29345. doi: 10.18632/oncotarget.16440

Figure 6. Z-LIG enhanced Ace-H3(lys9/14) expression and recruitment onto the ERα promoter in MDA-MB-231 cells.

Figure 6

(A-B) Expression of Ace-H3(lys9/14) and Total-Histone 3 was detected by Western blotting after MDA-MB-231 cells were treatment with Z-LIG as indicated for 72 h or Z-LIG (50 μM) for indicated times. (C) ChIP analysis showing recruitment of Ace-H3(lys9/14) onto ERpro315 of the ERα promoter. MDA-MB-231 cells were treated with Z-LIG (50 μM) for 72 h. DNA fragments that immunoprecipitated by normal IgG or anti-Ace-H3(lys9/14) antibodies were amplified by PCR using primers for ERpro315. The blots were a representative of three independent experiments.