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. 2017 Mar 22;8(17):29328–29345. doi: 10.18632/oncotarget.16440

Figure 8. Z-LIG decreased IFI16 and HDACs expression and recruitment onto the ERα promoter in MDA-MB-231.

Figure 8

(A) Expression of IFI16 and HDACs was detected by Western blotting in MDA-MB-231cells after treated with Z-LIG for 72 h. (B) ChIP analysis showing recruitment of IFI16 and HDACs onto ERpro315 of the ERα promoter. MDA-MB-231 cells were treated with Z-LIG (50 μM) for 72 h. DNA fragments that immunoprecipitated by normal IgG or or anti-IFI16, anti-HDAC1, anti-HDAC4/5/7, anti-HDAC2 antibodies antibodies were amplified by PCR using primers for ERpro315. (C-E) Expression of ERα was detected by Western blotting after MDA-MB-231 cells were transfected with vector and pcMV3-HDAC1, pcDNA3.1-HDAC7 or pcDNA-IFI16 for 24 h, andthen treated with Z-LIG (50 μM) for 72 h. β-actin was used as an internal control. The blots were a representative of three independent experiments.