Figure 3.

B cell and T cell responses induced by single immunization with Alum formulation. BALB/c mice were injected s.c. with 10 μg of gp41-54Q-GHC precipitated in Alum. dLNs were harvested at days 8, 12 and 18 post-challenge and cell suspensions stained with either PNA and anti-B220 mAb, or with anti-CD4, anti-CD44, anti-CXCR5 and anti-CD150 mAbs. Stained cells were analyzed by flow cytometry. Panel A shows the gating strategy used in flow cytometric analysis of B220^hiPNA^hi germinal center B cells from lymph nodes of BALB/c mice. Panel B shows the gating strategy used for selecting CD4⁺CD44^hiCXCR5⁺CD150^lo T follicular helper cells. GC B cell bar graphs (Panel C) represent the total number of B220⁺PNA^hi GC B cells per LN from mice at each time point. T_FH bar graphs (Panel D) represent the total number of CD4⁺CD44^hiCXCR5⁺CD150^lo T_FH cells per LN at each time point. Each bar represents mean±SEM. n = 4–6 mice at each time point. *Represents statistically significant differences between sample groups and background signal (p <0.05).