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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: J Biomed Nanotechnol. 2016 Jun;12(6):1303–1311. doi: 10.1166/jbn.2016.2242

Figure 5.

Figure 5

B cell and T cell responses induced by single immunization with polyanhydride nanovaccine. BALB/c mice were injected s.c. with 500 μg total of gp41-loaded 20:80 CPTEG:CPH nanovaccine. dLNs were harvested at days 8, 12 and 18 post-challenge and cell suspensions stained with either PNA and anti-B220 mAb, or with anti-CD4, anti-CD44, anti-CXCR5 and anti-CD150 mAbs. Stained cells were analyzed by flow cytometry. GC B cell bar graphs (Panel A) represent the total number of B220+PNAhi GC B cells per LN from mice at each time point. TFH bar graphs (Panel B) represent the total number of CD4+CD44hiCXCR5+CD150lo TFH cells per LN at each time point. Each bar represents mean±SEM. n = 4–6 mice at each time point. *Represents statistically significant differences between sample groups and background signal (p <0.05).