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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: J Biomed Nanotechnol. 2016 Jun;12(6):1303–1311. doi: 10.1166/jbn.2016.2242

Figure 7.

Figure 7

Serum antibody and B cell and T cell responses to multiple immunizations with polyanhydride nanovaccine. BALB/c mice were injected s.c. with 500 μg of antigen loaded 20:80 CPTEG:CPH nanoparticles on days 0, 7 and 14. Serum samples were collected at day 21 post-challenge and antibody titers (Panel A) were measured via ELISA. Plates were scanned at 450 nm. dLNs were harvested 7 days after the last challenge (day 21). Total numbers of GC B cells and TFH cells per LN (Panel B) were determined as described in the legend to Figure 3. Each bar represents mean±SEM. n = 4 mice at each time point. ^Represents statistically significant differences compared to Alum-treated groups at day 18. *Represents statistically significant differences between sample groups and background signal (p <0.05).