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. Author manuscript; available in PMC: 2017 Jun 7.
Published in final edited form as: J Allergy Clin Immunol. 2016 Nov 19;139(6):1897–1905.e1. doi: 10.1016/j.jaci.2016.10.018

Fig 3.

Fig 3

Characterization of rabbit antiserum induced by immunization with mCyp c 1. A, Titration of rabbit antiserum induced by immunization with mCyp c 1 for IgM, IgA, and IgG reactivity to rCyp c 1. Mean OD values of triplicates ± SD corresponding to bound IgM, IgA, and IgG (y-axis) are displayed for different serum dilutions (x-axis). B, Inhibition of IgE binding to rCyp c 1 by mCyp c 1–induced IgG in patients who are allergic to fish. For these patients, IgE binding (patients 1-8) (y-axis: mean OD values) to rCyp c 1, which was preincubated with various dilutions of serum from a rabbit obtained before (Pre) or after immunization with mCyp c 1 (x-axis), is shown. C, Epitope mapping of mCyp c 1–induced rabbit IgG antibodies. IgG reactivity (y-axis: mean OD values ± SDs) of mCyp c 1–specific IgG to rCyp c 1 and Cyp c 1 peptides 1 to 7 (x-axis). D, Cross-reactivity of mCyp c 1–induced rabbit IgG antibodies to nitrocellulose-blotted fish allergen extracts. Bound mCyp c 1–specific rabbit IgG antibodies were detected with 125I-labeled anti-rabbit IgG antibodies and visualized by auto-radiography. Molecular weights (kDa) are indicated on the left margin. Phl p 6, Recombinant Phl p 6.