Skip to main content
. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Matrix Biol. 2016 Nov 29;60-61:190–205. doi: 10.1016/j.matbio.2016.11.008

Figure 1. Collection and characterization of extracellular vesicles (EVs).

Figure 1

a) Subconfluent MDA MB-231 (MDAs) were subjected to serum starvation for 7–12 hours. Media was harvested, centrifuged to remove cell debris, and then filtered to enrich for EVs. This EV fraction was then used for subsequent experiments and analyses. Control media was prepared by placing serum-free media in the incubator and processing it similarly. b) Concentration of particles in the blank control media vs. ASC- and MDA-derived EVs as measured by NanoSight. ***p<0.001 vs. MDAs, ●● p<0.01 vs. ASCs, and ●●● p<0.001 vs. ASCs c) Size distribution of particles in the blank control media vs. ASC- and MDA-derived EVs as measured by NanoSight. d) Particle size distribution in blank control media vs. MDA-conditioned media as measured by Zetasizer.

HHS Vulnerability Disclosure