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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Matrix Biol. 2017 Jan 19;60-61:157–175. doi: 10.1016/j.matbio.2017.01.001

Fig 2.

Fig 2

FN fibril inhibition blocks the TGF-β pathway, inhibits cell size, cell number, and decreases migration in MCF10As. (A) Representative immunofluorescence images of Smad2 in MCF10As after 48 hours of treatment with TGF-β1 and FUD. (B) Immunofluorescence images of nuclei from part A (C) Quantification of nuclear colocalization of Smad2. N ≥ 16 for each condition. *p < 0.01 significantly different from TGF-β1, Student's t-test. (D) Quantification of the average cell size per condition. N ≥ 16 for each condition. *p < 0.01 significantly different from TGF-β1, Student's t-test. (E) Quantification of cell density. N ≥ 16 for each condition. *p < 0.01 significantly different from TGF-β1, Student's t-test. (F-G) Migration kinetics of MCF10A cells by continuous monitoring of live cell migration for approximately 48 hours. Direct comparison of FN fibril inhibitor &/or TGF-β1 effects on cell migration (F) after 24 hours, and (G) after 48 hours. Control samples served as the baseline cell index levels for comparison with wells containing a combination of FUD &/or TGF-β1. Cell index values from 4 wells per condition ± SD correspond to impedance from microelectrode sensor. **p < 0.05 significantly different from control or TGF-β1, Student's t-test. Scale bar is 50 μm.