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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Osteoarthritis Cartilage. 2016 Nov 22;25(6):943–951. doi: 10.1016/j.joca.2016.11.007

Figure 1. Expression of NR1D1 and BMAL1 mRNA and protein in cartilage.

Figure 1

(A) qPCR was performed on 14 normal and 14 OA human articular cartilage samples (* p<0.05, ** p<0.01). (B) NR1D1 and BMAL1 Protein expression was analyzed by immunohistochemistry. Representative images are shown for young normal, aged normal and OA normal appearing and OA lesion area. Images are representative of 5–8 samples for each tissue type. NR1D1 and BMAL1 positive cells were observed predominantly in the superficial and upper-mid zone of normal cartilage. Protein expression was reduced in OA cartilage even in the normal areas, although strong expression was observed in the cluster cells. (C) Immunohistochemistry of mouse cartilage. 6 mouse joints for each sample type were analyzed. Bar graphs indicate percentages of positive cells. NR1D1 and BMAL1 positive cells were distributed mainly in the superficial to upper-mid zone. Expression levels were significantly reduced with aging and with induction of surgical OA.