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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Osteoarthritis Cartilage. 2016 Nov 22;25(6):943–951. doi: 10.1016/j.joca.2016.11.007

Figure 3. Effect of NR1D1 and BMAL1 knock down on expression of NR1D1 and BMAL1.

Figure 3

(A) Western blotting was performed on cultured chondrocytes from 7 individual donors after siRNA transfection, collected at T12 and T24 after DEX synchronization. Effective knock down of both NR1D1 and BMAL1 was confirmed at both time points. Knocking down BMAL1 decreased the expression level of NR1D1, while knocking down NR1D1 had less effect on BMAL1 protein level. (B) qPCR was performed on samples after siRNA transfection, and RNA collected at time points as indicated. NR1D1 expression was reduced at all time points by knocking down NR1D1 or BMAL1, while still showing rhythmicity. BM AL1 expression was reduced and dysregulated by knocking down BMAL1, whereas knocking down NR1D1 resulted in increased expression levels of BMAL1 after T24.