Figure 3. Enhanced MEKK4-p38-Noxa pathway in basal-type breast cancer cells contributes to cystine-deprived necrosis.
(A). Immunoblotting of indicated proteins in a panel of luminal or basal type breast cancer cells.
(B). Cell cytotoxicity and survival of BT549 infected with shScr and two different shMEKK4 under indicated levels of cystine for 24 hours (n=4, p < 0.01).
(C). Immunoblotting of MEKK4, MKK3/6 phosphorylation and p38 phosphorylation in BT549 with shScr and shMEKK4 under high (HC, 200 μM) or low cystine (LC, 1 μM) for 18 hours.