Figure 3.

Slide set-up for culturing Aspiculuris tetraptera worms at room temperature. A square was drawn in the center of a 75 × 25 mm glass slide by using a wax pencil, and 6 to 8 drops of distilled water were placed within the square. The gravid female worms were placed in the distilled water and macerated to release eggs. Each slide with worms was placed on 2 wooden sticks in a Petri dish with folded Kimwipe saturated with water in the bottom.