Figure 3.
Mice in the FV phlebotomy group were more often inactive than all other groups. The number of inactive episodes, defined as being largely immobile or freezing for 15 s or more, was recorded for 10 min after the phlebotomy or sham procedures. Although the range varied from 0 to 8 inactive periods per mouse, the number of inactive periods observed per mouse (mean ± SEM) in the FV group was significantly greater than that in the other treatment groups. (one-way ANOVA with Tukey post hoc tests: F4,41 = 6.347, P < 0.001). No inactive periods were observed for any tail manipulation group, and only one inactive period was observed in the SF group. Error bars represent SEM. *, P < 0.05.