Fig. 2. (A) Scleroderma patient specimen sections are stained with 4′,6-diamidino-2-phenylindole (DAPI), periostin and α-smooth muscle actin (α-SMA). Periostin showed diffuse positivity through the whole dermis. α-SMA expression is mainly located at pericyte and is not colocalized with periostin expression. (B) Control specimen sections are stained with DAPI, periostin and α-SMA. Periostin showed focal positivity along dermo-epidermal junction and perivascular area. α-SMA expression is mainly located at pericyte and is somewhat colocalized with perivascular periostin positivity.