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Effects of GYF-21 on the expression of chemokines by microglia. BV-2 cells were pretreated with various concentrations of GYF-21 for 0.5 h and then stimulated with LPS (0.5 μg/ml) for 6 h (RT-PCR) or 12 h (ELISA). The mRNA transcription of MIP-1α and MCP-1 was determined by RT-PCR (A,B). The levels of MIP-1α and MCP-1 in the supernatants were determined by ELISA (C,D). Data are representative of three independent experiments. #P < 0.05 vs. Vehicle, ∗P < 0.05 vs. LPS.
