Figure 10.

AGO2 abolishes interaction between IRF3 and CBP/p300. (A) HEK293T cells were cotransfected with Myc-IBiD, Flag-IRF3, and different concentrations of HA-AGO2 (i.e., 0.5, 1, and 2 μg) with empty plasmid as control. After 36 h, cells were stimulated by SeV for 12 h, and cell proteins were extracted, and immunoprecipitation was performed with anti-HA, anti-Flag, and anti-Myc antibodies. (B) HEK293T cells were cotransfected with HA-AGO2 and Flag-IRF3 or Flag-IRF3-5D with empty vectors as control. After 36 h, cells were stimulated by SeV for 12 h, and cell lysates were immunoprecipitated with an anti-Flag antibody and immunoblotted with an anti-CBP antibody.