Figure 11.

AGO2 interacts with IRF3 by its MID domain. (A) HEK293T cells were co-transfected with four truncations of AGO2 and IRF3 and immunoprecipitation was performed at 36 h later. (B) The MID deleted mutation of AGO2 (HA-AGO2-MIDdel) and IRF3 were co-transfected into HEK293T cell. After 36 h, immunoprecipitation was performed. (C) HEK293T cells were co-transfected with HA–AGO2-MIDdel and Flag-IRF3 or Flag-IRF3-5D with empty vectors as control. After 36 h, cells were stimulated by SeV for 12 h and cell lysates were immunoprecipitated with an anti-Flag antibody and immunoblotted with an anti-CBP antibody.