Figure 5.

AGO2 does not affect activation of IRF3. (A) HEK293T cells were transfected with Flag-VISA and HA-AGO2 with empty vectors as control. After 36 h, cells were lysed, and total IRF3 and Ser386/Ser396 phosphorylated forms of IRF3 were detected by western blot. (B) HEK293T cells were transfected with Flag-VISA and HA-AGO2 or empty vectors. After 36 h, subcellular fractionation was implemented to separate the cytoplasm and nucleus from cells. Western blots were performed to detect distribution of total IRF3 and Ser386/Ser396 phosphorylated IRF3 in cells.