Figure 6.

AGO2 does not influence DNA binding ability of IRF3. HEK293T cells were transfected with Flag-IRF3 in the absence or presence of HA-AGO2, and cells were infected with SeV at 24 h post transfection. After 12 h, cells were lysed, and cell lysates were mixed with a biotinylated DNA probe (biotin-3 × ISRE or biotin-3 × ISRE mutant) and incubated for 1 h at 4 °C. Then, streptavidin beads were added and incubated overnight at 4°C. The beads were washed four times with PBS and resolved by 2 × SDS loading buffer for western blot.