Figure 7.

AGO2 interacts with IRF3 in HEK293T cells. (A) HEK293T cells were transfected with HA-AGO2 and Flag-IRF3 or with Flag-AGO2 and HA-IRF3. After 36 h, cells were lysed, and immunoprecipitation was performed. (B) HEK293T cells were stimulated with SeV for 3 h, and cell proteins were extracted. Anti-IRF3 antibody was used for immunoprecipitation. (C) HEK293 cells were mock infected or infected with SeV. At 24 h, subcellular localizations of IRF3 and AGO2 were visualized by immunofluorescence and microscopy.