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. 2017 May 22;8:103. doi: 10.3389/fendo.2017.00103

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Copyright © 2017 Arauchi, Matsuura, Shimizu and Okano.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Evaluation of thyroid specificity with characteristic thyroid markers on culture day 20 and measurement of free T₄. (A) Levels of thyroid-specific mRNA expression. Bars indicate average percentage of Gapdh gene expression ± SD (n = 3). ND, not-detected. (B) Immunostaining of embryoid bodies on day 20 with thyroid-specific markers NIS, thyroglobulin, and thyroid-stimulating hormone receptor. Images in the top line present negative controls. Scale bar = 50 µm in the top line, 30 µm in the lower lines. (C) Levels of human-free T₄ were higher significantly in the cultured-cell medium than in the control medium (*p < 0.01). Control medium is thyroid-stimulating hormone-supplemented medium before cells were harvested. Bars indicate average-free T₄ levels ± SD (n = 3). Statistical analysis was performed by unpaired Student’s t-test.

Evaluation of thyroid specificity with characteristic thyroid markers on culture day 20 and measurement of free T₄. (A) Levels of thyroid-specific mRNA expression. Bars indicate average percentage of Gapdh gene expression ± SD (n = 3). ND, not-detected. (B) Immunostaining of embryoid bodies on day 20 with thyroid-specific markers NIS, thyroglobulin, and thyroid-stimulating hormone receptor. Images in the top line present negative controls. Scale bar = 50 µm in the top line, 30 µm in the lower lines. (C) Levels of human-free T₄ were higher significantly in the cultured-cell medium than in the control medium (*p < 0.01). Control medium is thyroid-stimulating hormone-supplemented medium before cells were harvested. Bars indicate average-free T₄ levels ± SD (n = 3). Statistical analysis was performed by unpaired Student’s t-test.