Figure 4.

Luteinising hormone pulse analysis

JDV used a blinded deconvolution method with 93% sensitivity and specificity to analyse luteinising hormone pulsatility by calculating the number of luteinising hormone pulses (A), the mean amplitude of luteinising hormone pulses (B), and the orderliness of the pulses (approximate entropy; the lower the number the more ordered the pulses are, with zero denoting perfect orderliness; C). A generalised linear model was used for analysis with a Poisson error structure for number of pulses, and with a gamma structure for mean amplitude and orderliness of luteinising hormone pulses. A standard crossover analysis was implemented, with period, administration sequence, and treatment as fixed effects and subject as a random effect. Box plots: line, median; box, IQR; whiskers extend to the extremes of the data (minimum and maximum values).