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. 2017 Apr 19;198(11):4383–4393. doi: 10.4049/jimmunol.1600714

FIGURE 1.

FIGURE 1.

Influenza A infection increases AIM2 and stimulates dsDNA release into BAL. (A) Cultured human primary ATII cells and AMs from six donors were infected with influenza virus PR8 or MOCK control (Ctrl) at moi of 1, and RNA was extracted at 24 hpi for evaluation of AIM2 expression by real-time PCR (17, 18). Data show the relative expression of AIM2 and IL-1β mRNA normalized to the expression of human housekeeping gene GAPDH. (B–D) WT and AIM2−/− mice were challenged with 50LD50 of PR8 virus or DMEM control (Ctrl) intranasally. Lung tissues (B) were harvested at 3 dpi for evaluation of AIM2 and IL-1β expression by real-time RT-PCR. Data illustrate the relative expression of AIM2 normalized to mouse GAPDH. (C and D) Cell-free BALF was collected at 1 and 3 dpi for quantification of dsDNA (C) using QuantiFluor dsDNA kit (Promega) and analysis of DNA pattern (D) on Agilent High Sensitivity D1000 ScreenTape (Agilent). *p < 0.05, **p < 0.01, compared with the Ctrl group.