Fig. 3.

Overexpression of WT NS is toxic for NPCs upon neuronal differentiation. A. Time course of cell viability during neuronal differentiation analysed by MTT assay. NPCs were cultured and differentiated to neurons and cell viability was analysed at times 0 (proliferative conditions), 3 and 6 days after starting differentiation. Data are indicated as mean +/− SEM of three independent repeats, t-test: *p ≤ 0.05; ** p ≤ 0.01. Micrographs: bright field images of NPCs overexpressing WT and G392E NS at the end of the 6 days differentiation treatment. B. Percentage of ßIII-tubulin positive cells after differentiation of GFP, WT and G392E NS expressing NPCs for 6 days. Nuclei are stained with DAPI. Scale bar: 30 μm. Data are indicated as mean +/− SEM of three independent repeats (200 to 500 cells counted per condition in each repeat), t-test: **p ≤ 0.01; ***p ≤ 0.005.