Fig. 5.

Real-time RT-PCR analysis of selected genes in differentiated NPCs. NPCs stably overexpressing GFP or wild type (WT), G392E or delta NS (dNS) were differentiated for 6 days in vitro and their total RNA recovered for gene expression analysis by real-time RT-PCR using primer pairs targeting the indicated genes (see Supplementary Table 2). Data are shown as mean +/− SEM of the ratio between transgenic cultures and control (non-transfected, proliferating) NPCs of five independent repeats. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001, according to Student's t-test performed between GFP and NS cultures.

Real-time RT-PCR analysis of selected genes in differentiated NPCs. NPCs stably overexpressing GFP or wild type (WT), G392E or delta NS (dNS) were differentiated for 6 days in vitro and their total RNA recovered for gene expression analysis by real-time RT-PCR using primer pairs targeting the indicated genes (see Supplementary Table 2). Data are shown as mean +/− SEM of the ratio between transgenic cultures and control (non-transfected, proliferating) NPCs of five independent repeats. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001, according to Student's t-test performed between GFP and NS cultures.