Supplementary Fig. S3.

NPSC stably overexpressing GFP or wild type (WT), G392E or delta NS (dNS) were differentiated for 6 days in vitro and their total RNA recovered for gene expression analysis by real-time RT-PCR using primer pairs targeting the indicated genes. Data are shown as mean +/− SEM of the ratio between transgenic cultures and control (non-transfected) NPCs of five independent repeats. **p ≤ 0.01, according to Student's t-test performed between GFP and NS cultures. C. Expression levels in our RNA sequencing analysis of Eef1a and Rpl19, used in this study as reference genes for real-time RT-PCR experiments. Data are shown as normalised counts of reads for genes across the two cell lines. D. Catalase protein levels were determined in cell lysates of NPCs differentiated for six days by 10% SDS-PAGE followed by western blot with an anti-catalase specific antibody (upper panel). GAPDH (lower panel) in the same membrane was used as a loading control. Four independent repeats were used for densitometry analysis (histogram) showing no significant differences by non-parametric ANOVA (p = 0.561).