Fig. 3.

Long-term effect of treatment with ELN on the number of granule neurons of the dentate gyrus. A: Representative images of Hoechst-stained sections of the granule cell layer of an animal from each experimental group. The sides of the superimposed optical disector are 30 μm in length. The stars indicate individual nuclei. Note that nuclei intersecting the exclusion sides (thick lines) were not counted. B: Total number of granule cells in untreated and treated euploid and Ts65Dn mice (n = 6 for each experimental group). Values (mean ± SE) refer to one hemisphere. *p < 0.05; **p < 0.01 (Fisher LSD test). Abbreviations: ELN, ELND006; Eu, Euploid.