Fig. 4.

Short- and long-term effect of treatment with ELN on CTFs levels. Western blot analysis of CTFs in the hippocampus of treated and untreated Ts65Dn and euploid mice (n = 8 for each experimental group). P15 and P45 mice were treated with ELN in the period P3-P15. A, C: Examples of western blot images of CTFs in P15 (A) and P45 (C) mice. B, D: Quantification of CTFs levels in P15 (B) and P45 (D) mice normalized to GAPDH. Values (mean ± SE) are expressed as fold difference in comparison with untreated euploid mice. **p < 0.01; ***p < 0.001 (Fisher LSD test). Black asterisks in the gray bar indicate a difference between untreated Ts65Dn mice and treated euploid mice. White asterisks in the black bar indicate a difference between treated Ts65Dn mice and treated euploid mice. Abbreviations: ELN, ELND006; Eu, Euploid.