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. 2017 May 22;6:12. doi: 10.1186/s40035-017-0082-8

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — MANF did not upregulate HSP70 expression levels under H₂O₂ TG and TM treatment in SHSY-5Y cells. a Real-time PCR quantitation of HSP70 mRNA levels in control, H₂O₂ and H₂O₂ + MANF treatment group (mean ± SEM, n = 5). The relative mRNA expression levels of HSP70 in SHSY-5Y cells were normalized to those of β-actin. b Real-time PCR quantitation of HSP70 mRNA levels in control, TG and TG + MANF treatment group (mean ± SEM, n = 5). c Real-time PCR quantitation of HSP70 mRNA levels in control, TM and TM + MANF treatment group (mean ± SEM, n = 5). d Real-time PCR quantitation of HSP70 mRNA levels in control, 6-OHDA and 6-OHDA + MANF treatment group (mean ± SEM, n = 5). The relative mRNA expression levels of HSP70 in SHSY-5Y cells were normalized to those of β-actin