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. 2017 May 22;6:12. doi: 10.1186/s40035-017-0082-8

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Effects of shHSP70–3/−4 blockade on the protective effects of MANF against 6-OHDA induced apoptosis in SHSY-5Y cells. a, b SHSY-5Y cells were pretreated with control RNAi of shHSP70–3/−4 for 48 h and then stimulated with 50 μM 6-OHDA or 50 μM 6-OHDA + 4 μg/mL MANF for further 48 h. Cell viability was measured by TUNEL and MTT. c A representative Western blot result shows the expression of cleaved caspase-3 in control SHSY-5Y cells, SHSY-5Y cells treated with 150 μM 6-OHDA +4 μg/mL of MANF; 150 μM of 6-OHDA; A representative Western blot result shows the expression of cleaved caspase-3 in HSP70–3/−4RNAi SHSY-5Y cells treated with 150 μM 6-OHDA +4 μg/mL of MANF and 150 μM of 6-OHDA. (*p < 0.05; one-way ANOVA with Bonferroni post-tests)