Table 2.

PCR efficiency.

Patient/plate	Stored at −80 °C (weeks)	TCRβ identification (%)	TCRα identification (%)	Paired TCR chains (%)
Stored at −80 for N7 weeks
2/2	18.7	30/88 (34)	34/88 (39)	11/88 (13)
2/3	18.7	47/88 (53)	23/88 (26)	13/88 (15)
2/4	18.7	22/88 (25)	30/88 (34)	7/88 (8)
2/5	18.7	13/88 (15)	25/88 (28)	7/88 (8)
	Average	32%	32%	11%
Stored at −80 for >7 weeks
2/1	0.7	79/88 (90)	53/88 (60)	52/88 (59)
3/1	1.1	55/88 (63)	51/88 (58)	40/88 (45)
3/2	1.1	76/88 (86)	46/88 (52)	41/88 (47)
3/3	2.1	80/88 (91)	50/88 (57)	49/88 (56)
3/4	2.1	77/88 (88)	43/88 (49)	42/88 (48)
1/1	6.3	81/92 (88)	58/96 (60)	52/92 (57)
	Average	84%	56%	52%