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. 2017 Apr 20;14:86. doi: 10.1186/s12974-017-0856-z

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Acute ethanol changes BV2 microglial cytokine gene expression in vitro. Time course of cytokine expression in ethanol-treated BV2 cells: Microglia-like BV2 cells were treated with ethanol (85 mM) and the ethanol evaporated over time. Media ethanol concentrations (MECs) were measured (dashed line—left axis) and mRNA was collected from the BV2 cells. mRNA levels of a TNFα b Ccl2 c IL-1ra, and d IL-4 in ethanol-treated cells were normalized to mRNA levels in PBS-treated control cells (solid line—right axis). Data are presented as mean ± SEM. *p < 0.05. n = 4–6/group