Table 1.
List of primer sequences used in this study
18S F: | 5'-GGTAACCCGTTGAACCCCAT |
18S R: | 5'-CAACGCAAGCTTATGACCCG |
Iba1 F: | 5'-GGATTTGCAGGGAGGAAAAG |
Iba1 R: | 5'-TGGGATCATCGAGGAATTG |
CD68 F: | 5'-TGTCTGATCTTGCTAGGACCG |
CD68 R: | 5'-GAGAGTAACGGCCTTTTTGTGA |
TNFα F: | 5'-GACCCTCACACTCAGATCATCTTCT |
TNFα R: | 5'-CCTCCACTTGGTGGTTTGCT |
Ccl2 F: | 5'-CCAGCCTACTCATTGGGAT |
Ccl2 R: | 5'-GGGCCTGCTGTTCACAGTT |
IL-1ra F: | 5'- CTGTTGGCTGGCCTAATCCC |
IL-1ra R: | 5'-GCTTTGAACAAGCACCTGCC |
IL-4 F: | 5'-TGGGTCTCAACCCCCAGCTAGT |
IL-4 R: | 5'-TGCATGGCGTCCCTTCTCCTGT |
IL-10 F: | 5'-GCTCTTACTGACTGGCATGAG |
IL-10 R: | 5'-CGCAGCTCTAGGAGCATGTG |
Arg1 F: | 5'-TTAGGCCAAGGTGCTTGCTGCC |
Arg1 R: | 5'-TACCATGGCCCTGAGGAGGTTC |
CD11b F: | 5'-GAGGCCCCCAGGACTTTAAC |
CD11b R: | 5'-CTTCTTGGTGAGCGGGTTCT |
CX3CR1 F: | 5'-TCTTCACGTTCGGTCTGGTG |
CX3CR1 R: | 5'- TGCACTGTCCGGTTGTTCAT |
CSF1R F: | 5'-GTCCACGGCTCATGCTGAT |
CSF1R R: | 5'-GTGAGTACAGGCTCCCAAGAG |
CD86 F: | 5'-ACGATGGACCCCAGATGCACCA |
CD86 R: | 5'-GCGTCTCCACGGAAACAGCA |
CD206 F: | 5'-TCAGCTATTGGACGCGAGGCA |
CD206 R: | 5'-TCCGGGTTGCAAGTTGCCGT |
CD163 F: | 5'-CTCTGAATGACCCCCGAGGA |
CD163 R: | 5'-CACGGCACTCTTGGTTTGTG |
IL-1β F: | 5'-CTGGTGTGTGACGTTCCCATTA |
IL-1β R: | 5'-CCGACAGCACGAGGCTTT |
IL-6 F: | 5'-GGCCTTCCCTACTTCACAAG |
IL-6 R: | 5'-ATTTCCACGATTTCCCAGAG |
iNOS F: | 5'-GCTATGGCCGCTTTGATGTG |
iNOS R: | 5'-TCGAACTCCAATCTCGGTGC |
NOX2 F: | 5'-GGGAACTGGGCTGTGAATGA |
NOX2 R: | 5'-CAGTGCTGACCCAAGGAGTT |
TGFβ1 F: | 5'-CTCCCGTGGCTTCTAGTGC |
TGFβ1 R: | 5'-GCCTTAGTTTGGACAGGATCTG |
Ym1 F: | 5'-ACCCCTGCCTGTGTACTCACCT |
Ym1 R: | 5'-CACTGAACGGGGCAGGTCCAAA |
TLR2 F: | 5'-GCAAACGCTGTTCTGCTCAG |
TLR2 R: | 5'-AGGCGTCTCCCTCTATTGTATT |
TLR3 F: | 5'-TTGTCTTCTGCACGAACCTG |
TLR3 R: | 5'-GGCAACGCAAGGATTTTATT |
TLR4 F: | 5'-GCCTTTCAGGGAATTAAGCTCC |
TLR4 R: | 5'-AGATCAACCGATGGACGTGTAA |
TLR7 F: | 5'-ATGTGGACACGGAAGAGACAA |
TLR7 R: | 5'-GGTAAGGGTAAGATTGGTGGTG |
FasL F: | 5'-TTGAAAAGCAAATAGCCAACCC |
FasL R: | 5'-CACTCCAGAGATCAGAGCGG |
FasR F: | 5'-GCACCCTGACCCAGAATACC |
FasR R: | 5'-GTTCCATGTTCACACGAGTC |
NeuN F: | 5'-AACTTATGGAGCGGTCGTGT |
NeuN R: | 5'-GGCCGATGGTGTGATGGTAA |
MAP2 F: | 5'-GAGGAAGCAGCAAGTGGTGA |
MAP2 R: | 5'-GGGAGGATGGAGGAAGGTCT |
DCX F: | 5'-GACACCATGTGCTTAGGGCT |
DCX R: | 5'-ATTTGGGCAGTTTTCCCCCT |
GFAP F: | 5'-TCCTGGAACAGCAAAACAAG |
GFAP R: | 5'-CAGCCTCAGGTTGGTTTCAT |
S100β F: | 5'-CTAGGCATTCCCGTGAGCTG |
S100β R: | 5'-ATGAGCAACCTCTTCGGGTG |
MBP F: | 5'-CCCTCACGTTATTGTGGCGA |
MBP R: | 5'-AGACCTTCCAAAGAGCCCCA |
CNP F: | 5'-GACATAGTACCCGCAAAGGC |
CNP R: | 5'-AAGAGCTTGGGCAGGAATGT |
Pecam1 F: | 5'-GCATCGGCAAAGTGGTCAAG |
Pecam1 R: | 5'-TTGCTGGGTCATTGGAGGTC |
ICAM2 F: | 5'-ACTGGCACAGAGGAGATTGTG |
ICAM2 R: | 5'-AGGCTCCAGCAAGCAAAAGA |
CD200 F: | 5'-CCGAGAAGCTGGTGTCTAGC |
CD200 R: | 5'-ACCACTTCCACTTGAGCTGT |
CX3CL1 F: | 5'- CAACTTCCGAGGCACAGGAT |
CX3CL1 R: | 5'- CCAAACGGTGGTGGAGATGT |
c-Fos F: | 5'-TGGCACTAGAGACGGACAGA |
c-Fos R: | 5'-TTTCAACGCCGACTACGAGG |
EGR1 F: | 5'-GCGATGGTGGAGACGAGTTA |
EGR1 R: | 5'-AGAGGTCGGAGGATTGGTCA |
c-Jun F: | 5'-TGGGCACATCACCACTACAC |
c-Jun R: | 5'-TGACACTGGGAAGCGTGTTC |
Arc F: | 5'-CCAAGCCCCAGCTCCAATTA |
Arc R: | 5'-CCTACACACCCTATGCCAGC |
C3 F: | 5'-CAGGACGTGAGAGTCGATGG |
C3 R: | 5'-CTCTGCCTATGCTGCCTTCA |
C1qA F: | 5'-GAAGGGCGTGAAAGGCAATC |
C1qA R: | 5'-CAAGCGTCATTGGGTTCTGC |