Figure 3.

SLCP decreases Aβ42-induced ROS formation. N2a cells were treated with Aβ42 (10 μM) for 24 h in presence or absence of SLCP (1 μM) before being stained with CellROX oxidative stress reagents for detection of ROS levels. (a) ROS immunofluorescent (green) was increased by Aβ42, while Cur- and SLCP-treated cells decreased the green fluorescent signal. (b) Quantification of the fluorescent signals showed that Aβ42-treated cells had significantly increased (^∗p < 0.05; ^∗∗p < 0.01) ROS production, while SLCP prevented the Aβ42-induced increase in ROS more effectively than did dietary Cur. Scale bar indicates 100 μm and is applicable to all other images.